Roy Curtiss

Roy Curtiss,

Professor

Department: Department of Infectious Diseases & Immunology
Business Email: rcurtiss@ufl.edu

About Roy Curtiss

Expertise: Biotechnology, genetics, microbiology, immunology, vaccinology

Dr. Curtiss is a member of the National Academy of Sciences and Fellow of the American Academy of Microbiology, the American Association for the Advancement of Science, the St. Louis Academy of Sciences and the Arizona Arts, Science and Technology Academy. He has conducted research at Cornell University, Brookhaven National Laboratory, University of Chicago, Oak Ridge National Laboratory, University of Tennessee, University of Alabama at Birmingham, Washington University in St. Louis and Arizona State University.

Research Profile

Expertise: Biotechnology, genetics, microbiology, immunology, vaccinology

Dr. Curtiss is a member of the National Academy of Sciences and Fellow of the American Academy of Microbiology, the American Association for the Advancement of Science, the St. Louis Academy of Sciences and the Arizona Arts, Science and Technology Academy. He has conducted research at Cornell University, Brookhaven National Laboratory, University of Chicago, Oak Ridge National Laboratory, University of Tennessee, University of Alabama at Birmingham, Washington University in St. Louis and Arizona State University.

Dr. Curtiss’ early work was in avian genetics on cross breeding of White Plymouth Rock and White Cornish as a better broiler, genetics of bacteriophage P22 of Salmonella, mechanisms of bacterial conjugation in E. coli and use of bacterial minicells for studies of plasmids and cloned genes. Later work focused on establishing the molecular genetic bases for pathogenicity of Streptococcus mutans, Shigella flexneri, Mycobacterium leprae, Salmonella enterica, Escherichia coli pathovars and Bordatella avium. The Curtiss group developed biological containment first for cloned genes and then for recombinant attenuated Salmonella vaccines (RASVs) and most recently for cyanobacteria. The Curtiss lab developed the use of attenuated derivatives of pathogenic bacteria as vectors to deliver protective antigens encoded by genes from other pathogens. Dr. Curtiss also introduced use of transgenic plants as vaccine components and vectors.

Most recently members of the Curtiss lab group have contributed major technical innovations to more effectively deliver protective antigens and DNA vaccines by RASVs to stimulate all three branches of the immune system. This includes development of multiple methods for regulated delayed attenuation and regulated delayed synthesis of protective antigens to better enable vaccine strains to retain wild-type attributes at time of oral needle-free immunization. This will allow the vaccine to persist and to withstand host defenses, and efficiently evade effector lymphocytes before displaying complete attenuation to preclude induction of disease symptoms. The net result will be the synthesis and delivery of protective antigens to maximize induction of protective immunity. Safety of such vaccines and improvement of immunogenicity was achieved by developing the regulated delayed lysis in vivo attribute to confer complete biological containment and delivery of either protective antigens or DNA vaccines encoding them. These technologies are being used to address problems of global concern to design, construct and evaluate live RASVs to reduce morbidity and mortality caused by infectious disease agents of fish, poultry, swine, cattle and humans. Other efforts are directed at dealing with zoonotic threats and in developing preventative and therapeutic vaccines against cancer.

Publications

2021
Characterization of Brucella abortus S19 as a challenge strain for use in a mouse model of brucellosis.
Microbes and infection. 23(4-5) [DOI] 10.1016/j.micinf.2021.104809. [PMID] 33753207.
2021
Synthesis and delivery of Streptococcus pneumoniae capsular polysaccharides by recombinant attenuated Salmonella vaccines.
Proceedings of the National Academy of Sciences of the United States of America. 118(2) [DOI] 10.1073/pnas.2013350118. [PMID] 33380455.
2020
Pathogenicity and immunogenicity of Edwardsiella piscicida ferric uptake regulator (fur) mutations in zebrafish.
Fish & shellfish immunology. 107(Pt B):497-510 [DOI] 10.1016/j.fsi.2020.10.029. [PMID] 33176201.
2020
Regulating vitamin B12 biosynthesis via the cbiMCbl riboswitch in Propionibacterium strain UF1.
Proceedings of the National Academy of Sciences of the United States of America. 117(1):602-609 [DOI] 10.1073/pnas.1916576116. [PMID] 31836694.
2019
A Novel Cre Recombinase-Mediated In Vivo Minicircle DNA (CRIM) Vaccine Provides Partial Protection against Newcastle Disease Virus.
Applied and environmental microbiology. 85(14) [DOI] 10.1128/AEM.00407-19. [PMID] 31053588.
2019
A Recombinant Attenuated Yersinia pseudotuberculosis Vaccine Delivering a Y. pestis YopENt138-LcrV Fusion Elicits Broad Protection against Plague and Yersiniosis in Mice.
Infection and immunity. 87(10) [DOI] 10.1128/IAI.00296-19. [PMID] 31331960.
2019
Attenuated Salmonella Typhimurium expressing Salmonella Paratyphoid A O-antigen induces protective immune responses against two Salmonella strains.
Virulence. 10(1):82-96 [DOI] 10.1080/21505594.2018.1559673. [PMID] 31874075.
2019
Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803.
BMC biotechnology. 19(1) [DOI] 10.1186/s12896-019-0500-3. [PMID] 30630457.
2019
Correction to: characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803.
BMC biotechnology. 19(1) [DOI] 10.1186/s12896-019-0505-y. [PMID] 30760266.
2019
Regulated Delayed Shigella flexneri 2a O-antigen Synthesis in Live Recombinant Salmonella enterica Serovar Typhimurium Induces Comparable Levels of Protective Immune Responses with Constitutive Antigen Synthesis System.
Theranostics. 9(12):3565-3579 [DOI] 10.7150/thno.33046. [PMID] 31281498.
2018
Bi-valent polysaccharides of Vi capsular and O9 O-antigen in attenuated Salmonella Typhimurium induce strong immune responses against these two antigens.
NPJ vaccines. 3 [DOI] 10.1038/s41541-017-0041-5. [PMID] 29354293.
2018
Comparative evaluation of Salmonella Typhimurium vaccines derived from UK-1 and 14028S: Importance of inherent virulence.
PloS one. 13(9) [DOI] 10.1371/journal.pone.0203526. [PMID] 30192849.
2018
Conversion of RpoS- Attenuated Salmonella enterica Serovar Typhi Vaccine Strains to RpoS+ Improves Their Resistance to Host Defense Barriers.
mSphere. 3(1) [DOI] 10.1128/mSphere.00006-18. [PMID] 29507892.
2018
Salmonella Vaccines: Conduits for Protective Antigens.
Journal of immunology (Baltimore, Md. : 1950). 200(1):39-48 [DOI] 10.4049/jimmunol.1600608. [PMID] 29255088.
2017
Analysis of Spleen-Induced Fimbria Production in Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Strains.
mBio. 8(4) [DOI] 10.1128/mBio.01189-17. [PMID] 28830946.
2017
Evaluation of Escherichia Coli Isolates From Healthy Chickens to Determine Their Potential Risk to Poultry and Human Health
PLoS One. 12(7) [DOI] 10.1371/journal.pone.0180599. [PMID] 28671990.
2017
Evaluation of Recombinant Attenuated Salmonella Vaccine Strains for Broad Protection Against Extraintestinal Pathogenic Escherichia Coli
Frontiers in Immunology. 8 [DOI] 10.3389/fimmu.2017.01280. [PMID] 29062318.
2016
aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant.
mBio. 7(5) [DOI] 10.1128/mBio.01220-16. [PMID] 27601574.
2016
Multiple Antigens of Yersinia Pestis Delivered By Live Recombinant Attenuated Salmonella Vaccine Strains Elicit Protective Immunity Against Plague
Vaccine. 34(21):2410-2416 [DOI] 10.1016/j.vaccine.2016.03.094. [PMID] 27060051.
2016
Novel Vaccine Antigen Combinations Elicit Protective Immune Responses Against Escherichia Coli Sepsis
Vaccine. 34(5):656-662 [DOI] 10.1016/j.vaccine.2015.12.014. [PMID] 26707217.
2016
Optimizing Salmonella enterica serovar Typhimurium for bacteria-mediated tumor therapy.
Gut microbes. 7(2):171-7 [DOI] 10.1080/19490976.2016.1155021. [PMID] 26939530.
2015
A Live Oral Fowl Typhoid Vaccine with Reversible O-Antigen Production.
Avian diseases. 59(1):52-6 [PMID] 26292534.
View on: PubMed
2015
Correction for Santander et al., Fur-regulated iron uptake system of Edwardsiella ictaluri and its influence on pathogenesis and immunogenicity in the catfish host.
Infection and immunity. 83(12):4897-8 [DOI] 10.1128/IAI.01035-15. [PMID] 26556880.
2015
Efficiency of conditionally attenuated Salmonella enterica serovar Typhimurium in bacterium-mediated tumor therapy.
mBio. 6(2) [DOI] 10.1128/mBio.00254-15. [PMID] 25873375.
2015
Efficient generation of influenza virus with a mouse RNA polymerase I-driven all-in-one plasmid.
Virology journal. 12 [DOI] 10.1186/s12985-015-0321-5. [PMID] 26093583.
2015
Generation of influenza virus from avian cells infected by Salmonella carrying the viral genome.
PloS one. 10(3) [DOI] 10.1371/journal.pone.0119041. [PMID] 25742162.
2015
Oral administration of a recombinant attenuated Yersinia pseudotuberculosis strain elicits protective immunity against plague.
Vaccine. 33(48):6727-35 [DOI] 10.1016/j.vaccine.2015.10.074. [PMID] 26514425.
2015
Protection Against Necrotic Enteritis in Broiler Chickens by Regulated Delayed Lysis Salmonella Vaccines.
Avian diseases. 59(4):475-85 [DOI] 10.1637/11094-041715-Reg. [PMID] 26629620.
2015
Use of Ensure® nutrition shakes as an alternative formulation method for live recombinant Attenuated Salmonella Typhi vaccines.
BMC microbiology. 15 [DOI] 10.1186/s12866-015-0409-5. [PMID] 25879849.
2015
Zoonotic potential of Escherichia coli isolates from retail chicken meat products and eggs.
Applied and environmental microbiology. 81(3):1177-87 [DOI] 10.1128/AEM.03524-14. [PMID] 25480753.
2014
A low gastric pH mouse model to evaluate live attenuated bacterial vaccines.
PloS one. 9(1) [DOI] 10.1371/journal.pone.0087411. [PMID] 24489912.
2014
Assessment of attenuated Salmonella vaccine strains in controlling experimental Salmonella Typhimurium infection in chickens.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire. 78(1):23-30 [PMID] 24396177.
View on: PubMed
2014
Development of Streptococcus pneumoniae Vaccines Using Live Vectors.
Vaccines. 2(1):49-88 [PMID] 25309747.
View on: PubMed
2014
Evaluation of protective efficacy of live attenuated Salmonella enterica serovar Gallinarum vaccine strains against fowl typhoid in chickens.
Clinical and vaccine immunology : CVI. 21(9):1267-76 [DOI] 10.1128/CVI.00310-14. [PMID] 24990908.
2014
Evaluation of the prevalence and production of Escherichia coli common pilus among avian pathogenic E. coli and its role in virulence.
PloS one. 9(1) [DOI] 10.1371/journal.pone.0086565. [PMID] 24466152.
2014
Evaluation of YadC protein delivered by live attenuated Salmonella as a vaccine against plague.
Pathogens and disease. 70(2):119-31 [DOI] 10.1111/2049-632X.12076. [PMID] 23913628.
2014
In vivo IFN-γ secretion by NK cells in response to Salmonella typhimurium requires NLRC4 inflammasomes.
PloS one. 9(5) [DOI] 10.1371/journal.pone.0097418. [PMID] 24827856.
2014
Inflammatory effects of Edwardsiella ictaluri lipopolysaccharide modifications in catfish gut.
Infection and immunity. 82(8):3394-404 [DOI] 10.1128/IAI.01697-14. [PMID] 24866806.
2014
LcrV delivered via type III secretion system of live attenuated Yersinia pseudotuberculosis enhances immunogenicity against pneumonic plague.
Infection and immunity. 82(10):4390-404 [DOI] 10.1128/IAI.02173-14. [PMID] 25114109.
2014
Making Common Sense of Vaccines: An Example of Discussing the Recombinant Attenuated Salmonella Vaccine with the Public.
Nanoethics. 8:179-185 [PMID] 25152775.
View on: PubMed
2014
Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity.
International journal of medical microbiology : IJMM. 304(3-4):431-43 [DOI] 10.1016/j.ijmm.2014.02.006. [PMID] 24631214.
2014
Multiple effects of Escherichia coli Nissle 1917 on growth, biofilm formation, and inflammation cytokines profile of Clostridium perfringens type A strain CP4.
Pathogens and disease. 70(3):390-400 [DOI] 10.1111/2049-632X.12153. [PMID] 24532573.
2014
The delicate balance in genetically engineering live vaccines.
Vaccine. 32(35):4376-4385 [DOI] 10.1016/j.vaccine.2013.12.026. [PMID] 24370705.
2014
The route less taken: pulmonary models of enteric Gram-negative infection.
Pathogens and disease. 70(2):99-109 [DOI] 10.1111/2049-632X.12109. [PMID] 24259516.
2013
A colanic acid operon deletion mutation enhances induction of early antibody responses by live attenuated Salmonella vaccine strains.
Infection and immunity. 81(9):3148-62 [DOI] 10.1128/IAI.00097-13. [PMID] 23774599.
2013
A Phase I, dose-escalation trial in adults of three recombinant attenuated Salmonella Typhi vaccine vectors producing Streptococcus pneumoniae surface protein antigen PspA.
Vaccine. 31(42):4874-80 [DOI] 10.1016/j.vaccine.2013.07.049. [PMID] 23916987.
2013
Contribution of Thy1+ NK cells to protective IFN-γ production during Salmonella typhimurium infections.
Proceedings of the National Academy of Sciences of the United States of America. 110(6):2252-7 [DOI] 10.1073/pnas.1222047110. [PMID] 23345426.
2013
Evaluation of regulated delayed attenuation strategies for Salmonella enterica serovar Typhi vaccine vectors in neonatal and infant mice.
Clinical and vaccine immunology : CVI. 20(6):931-44 [DOI] 10.1128/CVI.00003-13. [PMID] 23616408.
2013
Export of extracellular polysaccharides modulates adherence of the Cyanobacterium synechocystis.
PloS one. 8(9) [DOI] 10.1371/journal.pone.0074514. [PMID] 24040267.
2013
Identification of an iron acquisition machinery in Flavobacterium columnare.
Diseases of aquatic organisms. 106(2):129-38 [DOI] 10.3354/dao02635. [PMID] 24113246.
2013
Low-pH rescue of acid-sensitive Salmonella enterica Serovar Typhi Strains by a Rhamnose-regulated arginine decarboxylase system.
Journal of bacteriology. 195(13):3062-72 [DOI] 10.1128/JB.00104-13. [PMID] 23645603.
2013
Natural lysogenization and transduction in Salmonella enterica serovar Choleraesuis by bacteriophage P1.
Research in microbiology. 164(1):1-5 [DOI] 10.1016/j.resmic.2012.09.004. [PMID] 22995628.
2013
New technologies in developing recombinant attenuated Salmonella vaccine vectors.
Microbial pathogenesis. 58:17-28 [DOI] 10.1016/j.micpath.2012.10.006. [PMID] 23142647.
2013
Pathogenicity of Yersinia pestis synthesis of 1-dephosphorylated lipid A.
Infection and immunity. 81(4):1172-85 [DOI] 10.1128/IAI.01403-12. [PMID] 23357387.
2013
Rapid, sensitive recovery of recombinant attenuated Salmonella enterica serovar Typhi vaccine strains from human blood.
Clinical and vaccine immunology : CVI. 20(9):1473-8 [DOI] 10.1128/CVI.00331-13. [PMID] 23885029.
2013
Sequencing and functional annotation of avian pathogenic Escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms.
Infection and immunity. 81(3):838-49 [DOI] 10.1128/IAI.00585-12. [PMID] 23275093.
2013
Utilizing Salmonella for antigen delivery: the aims and benefits of bacterial delivered vaccination.
Expert review of vaccines. 12(4):345-7 [DOI] 10.1586/erv.13.7. [PMID] 23560914.
2012
Amino acid substitutions in LcrV at putative sites of interaction with Toll-like receptor 2 do not affect the virulence of Yersinia pestis.
Microbial pathogenesis. 53(5-6):198-206 [DOI] 10.1016/j.micpath.2012.07.003. [PMID] 22841961.
2012
Comparative genome analysis of the high pathogenicity Salmonella Typhimurium strain UK-1.
PloS one. 7(7) [DOI] 10.1371/journal.pone.0040645. [PMID] 22792393.
2012
Fur-regulated iron uptake system of Edwardsiella ictaluri and its influence on pathogenesis and immunogenicity in the catfish host.
Infection and immunity. 80(8):2689-703 [DOI] 10.1128/IAI.00013-12. [PMID] 22615248.
2012
Live attenuated Salmonella vaccines against Mycobacterium tuberculosis with antigen delivery via the type III secretion system.
Infection and immunity. 80(2):798-814 [DOI] 10.1128/IAI.05525-11. [PMID] 22144486.
2012
Live attenuated Salmonella vaccines displaying regulated delayed lysis and delayed antigen synthesis to confer protection against Mycobacterium tuberculosis.
Infection and immunity. 80(2):815-31 [DOI] 10.1128/IAI.05526-11. [PMID] 22144485.
2012
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.
PloS one. 7(1) [DOI] 10.1371/journal.pone.0029481. [PMID] 22238616.
2012
NLRC4 inflammasomes in dendritic cells regulate noncognate effector function by memory CD8⁺ T cells.
Nature immunology. 13(2):162-9 [DOI] 10.1038/ni.2195. [PMID] 22231517.
2012
Phosphate groups of lipid A are essential for Salmonella enterica serovar Typhimurium virulence and affect innate and adaptive immunity.
Infection and immunity. 80(9):3215-24 [DOI] 10.1128/IAI.00123-12. [PMID] 22753374.
2012
The Asd(+)-DadB(+) dual-plasmid system offers a novel means to deliver multiple protective antigens by a recombinant attenuated Salmonella vaccine.
Infection and immunity. 80(10):3621-33 [DOI] 10.1128/IAI.00620-12. [PMID] 22868499.
2012
The Campylobacter jejuni Dps homologue is important for in vitro biofilm formation and cecal colonization of poultry and may serve as a protective antigen for vaccination.
Clinical and vaccine immunology : CVI. 19(9):1426-31 [DOI] 10.1128/CVI.00151-12. [PMID] 22787197.
2012
Thermorecovery of cyanobacterial fatty acids at elevated temperatures.
Journal of biotechnology. 161(4):445-9 [DOI] 10.1016/j.jbiotec.2012.08.013. [PMID] 22944207.
2012
Turning self-destructing Salmonella into a universal DNA vaccine delivery platform.
Proceedings of the National Academy of Sciences of the United States of America. 109(47):19414-9 [DOI] 10.1073/pnas.1217554109. [PMID] 23129620.
2012
Use of RapidChek® SELECT™ Salmonella to detect shedding of live attenuated Salmonella enterica serovar Typhi vaccine strains.
Journal of microbiological methods. 89(2):137-47 [DOI] 10.1016/j.mimet.2012.03.001. [PMID] 22425882.
2011
A live attenuated strain of Yersinia pestis KIM as a vaccine against plague.
Vaccine. 29(16):2986-98 [DOI] 10.1016/j.vaccine.2011.01.099. [PMID] 21320544.
2011
Comparison of a regulated delayed antigen synthesis system with in vivo-inducible promoters for antigen delivery by live attenuated Salmonella vaccines.
Infection and immunity. 79(2):937-49 [DOI] 10.1128/IAI.00445-10. [PMID] 21134969.
2011
Complete genome sequence of the universal killer Salmonella enterica Serovar Typhimurium UK-1 (ATCC 68169).
Journal of bacteriology. 193(15):4035-6 [DOI] 10.1128/JB.05224-11. [PMID] 21622747.
2011
Developing live vaccines against plague.
Journal of infection in developing countries. 5(9):614-27 [PMID] 21918302.
View on: PubMed
2011
Effect of deletion of genes involved in lipopolysaccharide core and O-antigen synthesis on virulence and immunogenicity of Salmonella enterica serovar typhimurium.
Infection and immunity. 79(10):4227-39 [DOI] 10.1128/IAI.05398-11. [PMID] 21768282.
2011
Immunization with Salmonella enterica serovar Typhimurium-derived outer membrane vesicles delivering the pneumococcal protein PspA confers protection against challenge with Streptococcus pneumoniae.
Infection and immunity. 79(2):887-94 [DOI] 10.1128/IAI.00950-10. [PMID] 21115718.
2011
Improving Salmonella vector with rec mutation to stabilize the DNA cargoes.
BMC microbiology. 11 [DOI] 10.1186/1471-2180-11-31. [PMID] 21303535.
2011
Lrp acts as both a positive and negative regulator for type 1 fimbriae production in Salmonella enterica serovar Typhimurium.
PloS one. 6(10) [DOI] 10.1371/journal.pone.0026896. [PMID] 22046399.
2011
Palmitoylation state impacts induction of innate and acquired immunity by the Salmonella enterica serovar typhimurium msbB mutant.
Infection and immunity. 79(12):5027-38 [DOI] 10.1128/IAI.05524-11. [PMID] 21930761.
2011
Phenotype, virulence and immunogenicity of Edwardsiella ictaluri cyclic adenosine 3′,5′-monophosphate receptor protein (Crp) mutants in catfish host.
Fish & shellfish immunology. 31(6):1142-53 [DOI] 10.1016/j.fsi.2011.10.009. [PMID] 22015784.
2011
Protective cellular responses elicited by vaccination with influenza nucleoprotein delivered by a live recombinant attenuated Salmonella vaccine.
Vaccine. 29(23):3990-4002 [DOI] 10.1016/j.vaccine.2011.03.066. [PMID] 21466806.
2011
Salmonella synthesizing 1-dephosphorylated [corrected] lipopolysaccharide exhibits low endotoxic activity while retaining its immunogenicity.
Journal of immunology (Baltimore, Md. : 1950). 187(1):412-23 [DOI] 10.4049/jimmunol.1100339. [PMID] 21632711.
2011
The impact of vaccines and vaccinations: Challenges and opportunities for modelers.
Mathematical biosciences and engineering : MBE. 8(1):77-93 [DOI] 10.3934/mbe.2011.8.77. [PMID] 21361401.
2011
Α1-giardin based live heterologous vaccine protects against Giardia lamblia infection in a murine model.
Vaccine. 29(51):9529-37 [DOI] 10.1016/j.vaccine.2011.09.126. [PMID] 22001876.
2010
Biogenesis of Yersinia pestis PsaA in recombinant attenuated Salmonella Typhimurium vaccine (RASV) strain.
FEMS microbiology letters. 302(2):106-13 [DOI] 10.1111/j.1574-6968.2009.01827.x. [PMID] 20002189.
2010
Characterization of the contribution to virulence of three large plasmids of avian pathogenic Escherichia coli chi7122 (O78:K80:H9).
Infection and immunity. 78(4):1528-41 [DOI] 10.1128/IAI.00981-09. [PMID] 20086082.
2010
Construction of recombinant attenuated Salmonella enterica serovar typhimurium vaccine vector strains for safety in newborn and infant mice.
Clinical and vaccine immunology : CVI. 17(3):354-62 [DOI] 10.1128/CVI.00412-09. [PMID] 20053874.
2010
Delivery of woodchuck hepatitis virus-like particle presented influenza M2e by recombinant attenuated Salmonella displaying a delayed lysis phenotype.
Vaccine. 28(41):6704-13 [DOI] 10.1016/j.vaccine.2010.07.083. [PMID] 20691653.
2010
Evaluation of Psn, HmuR and a modified LcrV protein delivered to mice by live attenuated Salmonella as a vaccine against bubonic and pneumonic Yersinia pestis challenge.
Vaccine. 29(2):274-82 [DOI] 10.1016/j.vaccine.2010.10.033. [PMID] 20979987.
2010
Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA.
Vaccine. 28(36):5810-6 [DOI] 10.1016/j.vaccine.2010.06.070. [PMID] 20600475.
2010
Fine-tuning synthesis of Yersinia pestis LcrV from runaway-like replication balanced-lethal plasmid in a Salmonella enterica serovar typhimurium vaccine induces protection against a lethal Y. pestis challenge in mice.
Infection and immunity. 78(6):2529-43 [DOI] 10.1128/IAI.00005-10. [PMID] 20308296.
2010
Immune responses to recombinant pneumococcal PsaA antigen delivered by a live attenuated Salmonella vaccine.
Infection and immunity. 78(7):3258-71 [DOI] 10.1128/IAI.00176-10. [PMID] 20479086.
2010
Immunogenicity of a live recombinant Salmonella enterica serovar typhimurium vaccine expressing pspA in neonates and infant mice born from naive and immunized mothers.
Clinical and vaccine immunology : CVI. 17(3):363-71 [DOI] 10.1128/CVI.00413-09. [PMID] 20053873.
2010
New technologies in using recombinant attenuated Salmonella vaccine vectors.
Critical reviews in immunology. 30(3):255-70 [PMID] 20370633.
View on: PubMed
2010
Production and secretion of fatty acids in genetically engineered cyanobacteria.
Proceedings of the National Academy of Sciences of the United States of America. [DOI] 10.1073/pnas.1001946107. [PMID] 20351245.
2010
Regulated delayed expression of rfc enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica vaccines.
Vaccine. 28(37):6094-103 [DOI] 10.1016/j.vaccine.2010.06.074. [PMID] 20599580.
2010
Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses.
Science (New York, N.Y.). 328(5986):1705-9 [DOI] 10.1126/science.1188454. [PMID] 20576892.
2010
Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium.
Journal of infection in developing countries. 4(11):723-31 [PMID] 21252450.
View on: PubMed
2010
Salmonella vaccine vectors displaying delayed antigen synthesis in vivo to enhance immunogenicity.
Infection and immunity. 78(9):3969-80 [DOI] 10.1128/IAI.00444-10. [PMID] 20605977.
2010
Yersinia pestis with regulated delayed attenuation as a vaccine candidate to induce protective immunity against plague.
Infection and immunity. 78(3):1304-13 [DOI] 10.1128/IAI.01122-09. [PMID] 20086087.
2009
A one-plasmid system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine.
Journal of virology. 83(18):9296-303 [DOI] 10.1128/JVI.00781-09. [PMID] 19587040.
2009
Evaluation of new generation Salmonella enterica serovar Typhimurium vaccines with regulated delayed attenuation to induce immune responses against PspA.
Proceedings of the National Academy of Sciences of the United States of America. 106(2):593-8 [DOI] 10.1073/pnas.0811697106. [PMID] 19114649.
2009
Innate immunity mediated by MyD88 signal is not essential for induction of lipopolysaccharide-specific B cell responses but is indispensable for protection against Salmonella enterica serovar Typhimurium infection.
Journal of immunology (Baltimore, Md. : 1950). 182(4):2305-12 [DOI] 10.4049/jimmunol.0801980. [PMID] 19201885.
2009
Leucine-responsive regulatory protein (Lrp) acts as a virulence repressor in Salmonella enterica serovar Typhimurium.
Journal of bacteriology. 191(4):1278-92 [DOI] 10.1128/JB.01142-08. [PMID] 19074398.
2009
Nickel-inducible lysis system in Synechocystis sp. PCC 6803.
Proceedings of the National Academy of Sciences of the United States of America. 106(51):21550-4 [DOI] 10.1073/pnas.0911953106. [PMID] 19995962.
2009
Oral vaccination with LcrV from Yersinia pestis KIM delivered by live attenuated Salmonella enterica serovar Typhimurium elicits a protective immune response against challenge with Yersinia pseudotuberculosis and Yersinia enterocolitica.
Vaccine. 27(39):5363-70 [DOI] 10.1016/j.vaccine.2009.06.078. [PMID] 19596407.
2009
PspA family fusion proteins delivered by attenuated Salmonella enterica serovar Typhimurium extend and enhance protection against Streptococcus pneumoniae.
Infection and immunity. 77(10):4518-28 [DOI] 10.1128/IAI.00486-09. [PMID] 19687204.
2009
Regulated delayed expression of rfaH in an attenuated Salmonella enterica serovar typhimurium vaccine enhances immunogenicity of outer membrane proteins and a heterologous antigen.
Infection and immunity. 77(12):5572-82 [DOI] 10.1128/IAI.00831-09. [PMID] 19805538.
2009
Salmonella enterica serovar typhimurium strains with regulated delayed attenuation in vivo.
Infection and immunity. 77(3):1071-82 [DOI] 10.1128/IAI.00693-08. [PMID] 19103774.
2009
The role of relA and spoT in Yersinia pestis KIM5 pathogenicity.
PloS one. 4(8) [DOI] 10.1371/journal.pone.0006720. [PMID] 19701461.
2009
The Salmonella Pathogenicity Island (SPI) 1 contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium.
BMC microbiology. 9 [DOI] 10.1186/1471-2180-9-3. [PMID] 19126220.
2008
A dual-signal regulatory circuit activates transcription of a set of divergent operons in Salmonella typhimurium.
Proceedings of the National Academy of Sciences of the United States of America. 105(52):20924-9 [DOI] 10.1073/pnas.0807071106. [PMID] 19091955.
2008
A sopB deletion mutation enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica vaccines.
Infection and immunity. 76(11):5238-46 [DOI] 10.1128/IAI.00720-08. [PMID] 18765737.
2008
Analysis of type II secretion of recombinant pneumococcal PspA and PspC in a Salmonella enterica serovar Typhimurium vaccine with regulated delayed antigen synthesis.
Infection and immunity. 76(7):3241-54 [DOI] 10.1128/IAI.01623-07. [PMID] 18458067.
2008
Contribution of the SitABCD, MntH, and FeoB metal transporters to the virulence of avian pathogenic Escherichia coli O78 strain chi7122.
Infection and immunity. 76(2):601-11 [PMID] 18025097.
View on: PubMed
2008
Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome.
Applied and environmental microbiology. 74(13):4241-5 [DOI] 10.1128/AEM.00940-08. [PMID] 18487404.
2008
Immunogenicity of recombinant attenuated Salmonella enterica serovar Typhimurium vaccine strains carrying a gene that encodes Eimeria tenella antigen SO7.
Infection and immunity. 76(12):5745-53 [DOI] 10.1128/IAI.00897-08. [PMID] 18809658.
2008
MyD88 signaling is not essential for induction of antigen-specific B cell responses but is indispensable for protection against Streptococcus pneumoniae infection following oral vaccination with attenuated Salmonella expressing PspA antigen.
Journal of immunology (Baltimore, Md. : 1950). 181(9):6447-55 [PMID] 18941235.
View on: PubMed
2008
Presence of wild-type and attenuated Salmonella enterica strains in brain tissues following inoculation of mice by different routes.
Infection and immunity. 76(7):3268-72 [DOI] 10.1128/IAI.00244-08. [PMID] 18474649.
2008
Recombinant attenuated Salmonella enterica serovar typhimurium expressing the carboxy-terminal domain of alpha toxin from Clostridium perfringens induces protective responses against necrotic enteritis in chickens.
Clinical and vaccine immunology : CVI. 15(5):805-16 [DOI] 10.1128/CVI.00457-07. [PMID] 18337376.
2008
Regulated programmed lysis of recombinant Salmonella in host tissues to release protective antigens and confer biological containment.
Proceedings of the National Academy of Sciences of the United States of America. 105(27):9361-6 [DOI] 10.1073/pnas.0803801105. [PMID] 18607005.
2008
Regulation of Vi capsular polysaccharide synthesis in Salmonella enterica serotype Typhi.
Journal of infection in developing countries. 2(6):412-20 [PMID] 19745516.
View on: PubMed
2008
SdiA, an N-acylhomoserine lactone receptor, becomes active during the transit of Salmonella enterica through the gastrointestinal tract of turtles.
PloS one. 3(7) [DOI] 10.1371/journal.pone.0002826. [PMID] 18665275.
2007
Oral vaccination with different antigens from Yersinia pestis KIM delivered by live attenuated Salmonella typhimurium elicits a protective immune response against plague.
Advances in experimental medicine and biology. 603:387-99 [PMID] 17966435.
View on: PubMed
2007
Role of RpoS in fine-tuning the synthesis of Vi capsular polysaccharide in Salmonella enterica serotype Typhi.
Infection and immunity. 75(3):1382-92 [PMID] 17178790.
View on: PubMed
2007
The introduction of new vaccines into developing countries. IV: Global Access Strategies.
Vaccine. 25(20):4003-11 [PMID] 17363119.
View on: PubMed
2006
A recombinant attenuated Salmonella enterica serovar Typhimurium vaccine encoding Eimeria acervulina antigen offers protection against E. acervulina challenge.
Infection and immunity. 74(12):6785-96 [PMID] 16982843.
View on: PubMed
2006
Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains.
FEMS microbiology letters. 265(1):81-8 [PMID] 17034415.
View on: PubMed
2005
Inactivation of the pst system reduces the virulence of an avian pathogenic Escherichia coli O78 strain.
Infection and immunity. 73(7):4138-45 [PMID] 15972503.
View on: PubMed
2005
Selective capture of Salmonella enterica serovar typhi genes expressed in macrophages that are absent from the Salmonella enterica serovar Typhimurium genome.
Infection and immunity. 73(8):5217-21 [PMID] 16041043.
View on: PubMed
2005
Virulence attenuation and live vaccine potential of aroA, crp cdt cya, and plasmid-cured mutants of Salmonella enterica serovar Abortusovis in mice and sheep.
Infection and immunity. 73(7):4302-8 [PMID] 15972523.
View on: PubMed
2004
Immunogenicity against human papillomavirus type 16 virus-like particles is strongly enhanced by the PhoPc phenotype in Salmonella enterica serovar Typhimurium.
Infection and immunity. 72(2):750-6 [PMID] 14742517.
View on: PubMed
2003
Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain.
Proceedings of the National Academy of Sciences of the United States of America. 100(1):247-52 [PMID] 12506201.
View on: PubMed
2003
Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization.
FEMS immunology and medical microbiology. 37(2-3):99-104 [PMID] 12832112.
View on: PubMed
2003
Role of avian pathogenic Escherichia coli virulence factors in bacterial interaction with chicken heterophils and macrophages.
Infection and immunity. 71(1):494-503 [PMID] 12496200.
View on: PubMed
2003
Role of virulence factors in resistance of avian pathogenic Escherichia coli to serum and in pathogenicity.
Infection and immunity. 71(1):536-40 [PMID] 12496207.
View on: PubMed
2002
Bacterial infectious disease control by vaccine development.
The Journal of clinical investigation. 110(8):1061-6 [PMID] 12393839.
View on: PubMed
2002
Construction of transcriptional and translational lacZ gene reporter plasmids for use in Streptococcus mutans.
Journal of microbiological methods. 49(2):163-71 [PMID] 11830302.
View on: PubMed
2002
Cruzipain induces both mucosal and systemic protection against Trypanosoma cruzi in mice.
Infection and immunity. 70(9):5065-74 [PMID] 12183554.
View on: PubMed
2002
Immune responses to recombinant pneumococcal PspA antigen delivered by live attenuated Salmonella enterica serovar typhimurium vaccine.
Infection and immunity. 70(4):1739-49 [PMID] 11895935.
View on: PubMed
2002
Transduction-mediated transfer of unmarked deletion and point mutations through use of counterselectable suicide vectors.
Journal of bacteriology. 184(1):307-12 [PMID] 11741872.
View on: PubMed
1992
Cloning, sequence analysis, and expression in Escherichia coli of a streptococcal plasmin receptor.
Journal of bacteriology. 174(16):5204-10 [PMID] 1322883.
View on: PubMed

Grants

Jan 2021 ACTIVE
Self-Destructing Salmonella as Innate Immunity Activator to Improve Food Safety
Role: Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Feb 2020 ACTIVE
Edwardsiella piscicida DNA vaccine vector to prevent Tilapia Lake Virus (TiLV) infection and develop generalized vaccine vectors to protect commercial fish from multiple infectious diseases to improve aquaculture productivity
Role: Principal Investigator
Funding: FOUNDATION FOR FOOD AND AGRICULTURE RES
Aug 2019 – Jul 2020
Design, construct and validate Salmonella Choleraesuis vaccine and vaccine vector strain with regulated delayed attenuation, regulated delayed synthesis of protective antigens, and regulated delayed lysis in vivo phenotypes
Role: Principal Investigator
Funding: CURTISS HEALTHCARE INC
Jul 2018 ACTIVE
Edwardsiella piscicida: A vaccine delivery platform for multiple fish pathogens.
Role: Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Jan 2018 – Mar 2019
Brucellosis Vaccine Prize: Phase 1
Role: Principal Investigator
Funding: GALVMED
Nov 2017 – Nov 2018
Recombinant immunocontraceptive vaccines for management of cat and dog populations
Role: Principal Investigator
Funding: UF FOU
Sep 2017 – Aug 2019
Recombinant immunocontraceptive vaccines to control rodent and ultimately other wildlife species.
Role: Principal Investigator
Funding: DIETRICH W BOTSTIBER FOU
Apr 2017 ACTIVE
A Food Safety Vaccine to Control Salmonella Enteritidis and Reduce Campylobacter in Poultry
Role: Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Jan 2017 – Dec 2019
Manipulate cell surface by triple sugars for broad-spectrum Salmonella vaccine development
Role: Other
Funding: NATL INST OF HLTH NIAID
Feb 2016 – Jul 2017
Disease prevention in Gallus domesticus, Sus scrofa and Bos taurus
Role: Principal Investigator
Funding: CURTISS HEALTHCARE INC
Oct 2015 – Jan 2021
Heterologous polysaccharide synthesis in attenuated Salmonella
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Aug 2015 – May 2020
Recombinant Attenuated Salmonella Vaccines for Humans
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Aug 2015 – Apr 2017
Recombinant Attenuated bacterial vaccines against
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID

Education

PhD
1962 · University of Chicago

Teaching Profile

Courses Taught
2018
GMS7980 Research for Doctoral Dissertation
2018
VME7979 Advanced Research
2018
VME6464 Molecular Pathogenesis
2017
VME6934 Topics in Veterinary Medical Sciences

Contact Details

Emails:
Business:
rcurtiss@ufl.edu