Astra Dinculescu

Astra Dinculescu,

Assistant Professor

Department: MD-OPHTHALMOLOGY
Business Phone: (352) 273-7548
Business Email: astra@ufl.edu

Publications

2020
Clarin-1 expression in adult mouse and human retina highlights a role of Müller glia in Usher syndrome.
The Journal of pathology. 250(2):195-204 [DOI] 10.1002/path.5360. [PMID] 31625146.
2019
Long-Term Effects of Gene Therapy in a Novel Mouse Model of Human MFRP-Associated Retinopathy.
Human gene therapy. 30(5):632-650 [DOI] 10.1089/hum.2018.192. [PMID] 30499344.
2019
Systemic Delivery of Genes to Retina Using Adeno-Associated Viruses.
Advances in experimental medicine and biology. 1185:109-112 [DOI] 10.1007/978-3-030-27378-1_18. [PMID] 31884597.
2018
Co-Expression of Wild-Type and Mutant S163R C1QTNF5 in Retinal Pigment Epithelium.
Advances in experimental medicine and biology. 1074:61-66 [DOI] 10.1007/978-3-319-75402-4_8. [PMID] 29721928.
2018
Cone Phosphodiesterase-6γ’ Subunit Augments Cone PDE6 Holoenzyme Assembly and Stability in a Mouse Model Lacking Both Rod and Cone PDE6 Catalytic Subunits.
Frontiers in molecular neuroscience. 11 [DOI] 10.3389/fnmol.2018.00233. [PMID] 30038560.
2017
Gene Therapy in a Large Animal Model of PDE6A-Retinitis Pigmentosa.
Frontiers in neuroscience. 11 [DOI] 10.3389/fnins.2017.00342. [PMID] 28676737.
2017
Modeling and Preventing Progressive Hearing Loss in Usher Syndrome III.
Scientific reports. 7(1) [DOI] 10.1038/s41598-017-13620-9. [PMID] 29044151.
2016
AAV-Mediated Clarin-1 Expression in the Mouse Retina: Implications for USH3A Gene Therapy.
PloS one. 11(2) [DOI] 10.1371/journal.pone.0148874. [PMID] 26881841.
2015
Pathological Effects of Mutant C1QTNF5 (S163R) Expression in Murine Retinal Pigment Epithelium.
Investigative ophthalmology & visual science. 56(11):6971-80 [DOI] 10.1167/iovs.15-17166. [PMID] 26513502.
2015
Stability and Safety of an AAV Vector for Treating RPGR-ORF15 X-Linked Retinitis Pigmentosa.
Human gene therapy. 26(9):593-602 [DOI] 10.1089/hum.2015.035. [PMID] 26076799.
2014
Gene therapy in the rd6 mouse model of retinal degeneration.
Advances in experimental medicine and biology. 801:711-8 [DOI] 10.1007/978-1-4614-3209-8_89. [PMID] 24664762.
2014
Tyrosine capsid-mutant AAV vectors for gene delivery to the canine retina from a subretinal or intravitreal approach.
Gene therapy. 21(1):96-105 [DOI] 10.1038/gt.2013.64. [PMID] 24225638.
2013
Cone phosphodiesterase-6α’ restores rod function and confers distinct physiological properties in the rod phosphodiesterase-6β-deficient rd10 mouse.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(29):11745-53 [DOI] 10.1523/JNEUROSCI.1536-13.2013. [PMID] 23864662.
2013
Review: the history and role of naturally occurring mouse models with Pde6b mutations.
Molecular vision. 19:2579-89 [PMID] 24367157.
View on: PubMed
2013
Targeting Tumor Hla Expression To Increase the Immunogenicity of Cancer Cells
Human Gene Therapy. 24(12)
2012
AAV-mediated gene therapy in mouse models of recessive retinal degeneration.
Current molecular medicine. 12(3):316-30 [PMID] 22300136.
View on: PubMed
2012
Gene therapy for retinitis pigmentosa caused by MFRP mutations: human phenotype and preliminary proof of concept.
Human gene therapy. 23(4):367-76 [DOI] 10.1089/hum.2011.169. [PMID] 22142163.
2012
Tyrosine-mutant AAV8 delivery of human MERTK provides long-term retinal preservation in RCS rats.
Investigative ophthalmology & visual science. 53(4):1895-904 [DOI] 10.1167/iovs.11-8831. [PMID] 22408006.
2011
Alternative splice variants of the USH3A gene Clarin 1 (CLRN1).
European journal of human genetics : EJHG. 19(1):30-5 [DOI] 10.1038/ejhg.2010.140. [PMID] 20717163.
2011
Long-term retinal function and structure rescue using capsid mutant AAV8 vector in the rd10 mouse, a model of recessive retinitis pigmentosa.
Molecular therapy : the journal of the American Society of Gene Therapy. 19(2):234-42 [DOI] 10.1038/mt.2010.273. [PMID] 21139570.
2011
Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina.
Molecular therapy : the journal of the American Society of Gene Therapy. 19(2):293-301 [DOI] 10.1038/mt.2010.234. [PMID] 21045809.
2011
Quantifying transduction efficiencies of unmodified and tyrosine capsid mutant AAV vectors in vitro using two ocular cell lines.
Molecular vision. 17:1090-102 [PMID] 21552473.
View on: PubMed
2009
Clarin-1, encoded by the Usher Syndrome III causative gene, forms a membranous microdomain: possible role of clarin-1 in organizing the actin cytoskeleton.
The Journal of biological chemistry. 284(28):18980-93 [DOI] 10.1074/jbc.M109.003160. [PMID] 19423712.
2009
Dose-Dependent Humoral Immune Responses To Intravitreal Delivery of Aav Vectors and Strategies To Circumvent
Molecular Therapy. 17
2009
Functional interchangeability of rod and cone transducin alpha-subunits.
Proceedings of the National Academy of Sciences of the United States of America. 106(42):17681-6 [DOI] 10.1073/pnas.0901382106. [PMID] 19815523.
2009
High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors.
Molecular therapy : the journal of the American Society of Gene Therapy. 17(3):463-71 [DOI] 10.1038/mt.2008.269. [PMID] 19066593.
2008
AAV-mediated gene therapy for retinal degeneration in the rd10 mouse containing a recessive PDEbeta mutation.
Investigative ophthalmology & visual science. 49(10):4278-83 [DOI] 10.1167/iovs.07-1622. [PMID] 18586879.
2008
Downregulation of p22phox in retinal pigment epithelial cells inhibits choroidal neovascularization in mice.
Molecular therapy : the journal of the American Society of Gene Therapy. 16(10):1688-94 [DOI] 10.1038/mt.2008.164. [PMID] 18665154.
2008
Intraocular route of AAV2 vector administration defines humoral immune response and therapeutic potential.
Molecular Vision. 14 [PMID] 18836574.
View on: PubMed
2008
Intraocular route of AAV2 vector administration defines humoral immune response and therapeutic potential.
Molecular vision. 14:1760-9 [PMID] 18836574.
View on: PubMed
2007
Anti-clarin-1 AAV-delivered ribozyme induced apoptosis in the mouse cochlea.
Hearing research. 230(1-2):9-16 [PMID] 17493778.
View on: PubMed
2006
Arrestin translocation in rod photoreceptors.
Advances in experimental medicine and biology. 572:455-64 [PMID] 17249609.
View on: PubMed
2006
Disease mechanisms and gene therapy in a mouse model for X-linked retinoschisis.
Advances in experimental medicine and biology. 572:283-9 [PMID] 17249585.
View on: PubMed
2005
Adeno-associated virus-mediated expression of vascular endothelial growth factor peptides inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathy.
Human gene therapy. 16(11):1247-54 [PMID] 16259558.
View on: PubMed
2005
Adeno-associated virus-vectored gene therapy for retinal disease.
Human gene therapy. 16(6):649-63 [PMID] 15960597.
View on: PubMed
2005
Prolonged recovery of retinal structure/function after gene therapy in an Rs1h-deficient mouse model of x-linked juvenile retinoschisis.
Molecular therapy : the journal of the American Society of Gene Therapy. 12(4):644-51 [PMID] 16027044.
View on: PubMed
2004
The surface of visual arrestin that binds to rhodopsin.
Molecular vision. 10:392-8 [PMID] 15215746.
View on: PubMed
2002
Insertional mutagenesis and immunochemical analysis of visual arrestin interaction with rhodopsin.
The Journal of biological chemistry. 277(14):11703-8 [PMID] 11809770.
View on: PubMed
1993
The effect of dihydronicotinate N-substitution on the brain-targeting efficacy of a zidovudine chemical delivery system.
Pharmaceutical research. 10(9):1356-62 [PMID] 8234177.
View on: PubMed

Grants

Jun 2021 ACTIVE
Generation and Characterization of Swine Models of Usher Syndrome Type 3
Role: Principal Investigator
Funding: FOU FOR FIGHTING BLINDNESS
Sep 2019 ACTIVE
Transcriptional control of proteostasis in photoreceptors
Role: Co-Investigator
Funding: NATL INST OF HLTH NEI
Jul 2017 – Aug 2019
The role of basal C1QTNF5-S163R mutant deposits in drusen formation: implications for AMD
Role: Principal Investigator
Funding: BRIGHTFOCUS FOU
Sep 2016 ACTIVE
CLARIN 1 RETINAL FUNCTION AND THERAPEUTIC IMPLICATIONS FOR USH3
Role: Principal Investigator
Funding: NATL INST OF HLTH NEI

Teaching Profile

Courses Taught
2020-2021
GMS6009 Principles of Drug Action and Therapeutics
2017-2020
GMS6791 Visual Neuroscience Journal Club
2021
GMS6070 Sensory and Motor Systems

Contact Details

Phones:
Business:
(352) 273-7548
Emails:
Business:
astra@ufl.edu