James B Flanegan,
Professor Emeritus
Department:
Molecular Genetics & Microbiology
Business Phone:
(352) 294-8384
Business Email:
flanegan@ufl.edu
Teaching Profile
Courses Taught
2013-2017,2019-2023
GMS6034 Advanced Virology I: Genetics and RNA
2013-2017,2019-2023
GMS6035 Advanced Virology II: RNA Viruses
2014-2019,2021-2023
BCH6415 Advanced Molecular and Cell Biology
2018
GMS7980 Research for Doctoral Dissertation
2018
GMS7979 Advanced Research
2015
BCH4905 Biochemistry Senior Research
2023-2024
BCH5413 Mammalian Molecular Biology and Genetics
Publications
2015
Viral precursor protein P3 and its processed products perform discrete and essential functions in the poliovirus RNA replication complex.
Virology.
485:492-501
[DOI] 10.1016/j.virol.2015.07.018.
[PMID] 26303005.
2014
Amiloride inhibits the initiation of Coxsackievirus and poliovirus RNA replication by inhibiting VPg uridylylation.
Virology.
464-465:87-97
[DOI] 10.1016/j.virol.2014.06.025.
[PMID] 25058507.
2011
Non-template functions of viral RNA in picornavirus replication.
Current opinion in virology.
1(5):339-46
[DOI] 10.1016/j.coviro.2011.09.005.
[PMID] 22140418.
2010
The 5’CL-PCBP RNP complex, 3′ poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA.
Virology.
397(1):14-22
[DOI] 10.1016/j.virol.2009.11.006.
[PMID] 19945132.
2009
Functional role of the 5′ terminal cloverleaf in Coxsackievirus RNA replication.
Virology.
393(2):238-49
[DOI] 10.1016/j.virol.2009.07.039.
[PMID] 19732932.
2008
Protein-RNA tethering: the role of poly(C) binding protein 2 in poliovirus RNA replication.
Virology.
374(2):280-91
[DOI] 10.1016/j.virol.2007.12.039.
[PMID] 18252259.
2006
2Apro is a multifunctional protein that regulates the stability, translation and replication of poliovirus RNA.
Virology.
345(2):346-57
[PMID] 16297952.
2006
Relationship between poliovirus negative-strand RNA synthesis and the length of the 3′ poly(A) tail.
Virology.
345(2):509-19
[PMID] 16297425.
2005
3′-Terminal sequence in poliovirus negative-strand templates is the primary cis-acting element required for VPgpUpU-primed positive-strand initiation.
Journal of virology.
79(6):3565-77
[PMID] 15731251.
2004
Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis of acute myeloid leukemia.
Proceedings of the National Academy of Sciences of the United States of America.
101(14):4924-9
[PMID] 15044690.
2003
Initiation of poliovirus negative-strand RNA synthesis requires precursor forms of p2 proteins.
Journal of virology.
77(2):1075-83
[PMID] 12502823.
2003
Poliovirus cre(2C)-dependent synthesis of VPgpUpU is required for positive- but not negative-strand RNA synthesis.
Journal of virology.
77(9):5136-44
[PMID] 12692216.
2001
5′ cloverleaf in poliovirus RNA is a cis-acting replication element required for negative-strand synthesis.
The EMBO journal.
20(6):1439-48
[PMID] 11250909.
1999
Translating ribosomes inhibit poliovirus negative-strand RNA synthesis.
Journal of virology.
73(12):10104-12
[PMID] 10559325.
1997
Poliovirus RNA recombination in cell-free extracts.
RNA (New York, N.Y.).
3(6):624-33
[PMID] 9174097.
1997
Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C.
Journal of virology.
71(11):8482-9
[PMID] 9343205.
1996
Assays for poliovirus polymerase, 3D(Pol), and authentic RNA replication in HeLa S10 extracts.
Methods in enzymology.
275:35-57
[PMID] 9026649.
1996
Poliovirus RNA polymerase mutation 3D-M394T results in a temperature-sensitive defect in RNA synthesis.
Virology.
217(2):459-69
[PMID] 8610437.
1995
Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
Journal of virology.
69(9):5516-27
[PMID] 7636997.
1993
Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus.
Journal of virology.
67(2):822-31
[PMID] 8380467.
1992
Determination of the poliovirus RNA polymerase error frequency at eight sites in the viral genome.
Journal of virology.
66(6):3784-93
[PMID] 1316481.
1992
Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells.
Journal of virology.
66(11):6480-8
[PMID] 1328676.
1989
A conserved base pair within helix P4 of the Tetrahymena ribozyme helps to form the tertiary structure required for self-splicing.
The EMBO journal.
8(11):3391-9
[PMID] 2684642.
1989
A point mutation in the poliovirus polymerase gene determines a complementable temperature-sensitive defect of RNA replication.
Virology.
168(2):302-11
[PMID] 2536984.
1989
Interference with vaccinia virus growth caused by insertion of the coding sequence for poliovirus protease 2A.
Virology.
173(2):509-21
[PMID] 2556841.
1989
Self-catalyzed linkage of poliovirus terminal protein VPg to poliovirus RNA.
Cell.
59(3):511-9
[PMID] 2553272.
1988
Direct measurement of the poliovirus RNA polymerase error frequency in vitro.
Journal of virology.
62(2):558-62
[PMID] 2826815.
1988
Measurement of poliovirus RNA polymerase binding to poliovirion and nonviral RNAs using a filter-binding assay.
Nucleic acids research.
16(21):10339-52
[PMID] 2461545.
1987
Characterization of product RNAs synthesized in vitro by poliovirus RNA polymerase purified by chromatography on hydroxylapatite or poly(U) Sepharose.
Journal of virology.
61(2):611-4
[PMID] 3027390.
1986
Anti-VPg antibody precipitation of product RNA synthesized in vitro by the poliovirus polymerase and host factor is mediated by VPg on the poliovirion RNA template.
Journal of virology.
58(3):715-23
[PMID] 3009884.
1985
Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro.
Journal of virology.
54(2):256-64
[PMID] 2985794.
1982
Genome-length copies of poliovirion RNA are synthesized in vitro by the poliovirus RNA-dependent RNA polymerase.
The Journal of biological chemistry.
257(8):4610-7
[PMID] 6279640.
1982
Poliovirus RNA-dependent RNA polymerase synthesizes full-length copies of poliovirion RNA, cellular mRNA, and several plant virus RNAs in vitro.
Journal of virology.
44(1):209-16
[PMID] 6183446.
1980
Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.
Journal of virology.
35(3):732-40
[PMID] 6252335.
1979
Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.
Journal of virology.
32(1):155-61
[PMID] 232168.
1979
Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.
Journal of virology.
29(1):352-60
[PMID] 219230.
1977
5′-Terminal nucleotide sequences of polio virus polyribosomal RNA and virion RNA are identical.
Nature.
268(5617):270-2
[PMID] 196211.
1977
Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus.
Proceedings of the National Academy of Sciences of the United States of America.
74(3):961-5
[PMID] 191841.
1977
Inhibitory effect of agents altering the structure of DNA on the synthesis of pyrimidine deoxyribonucleotides in bacteriophage T4 DNA replication.
The Journal of biological chemistry.
252(17):6031-7
[PMID] 330522.
1977
Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).
Proceedings of the National Academy of Sciences of the United States of America.
74(9):3677-80
[PMID] 198796.
1977
Regulation of deoxyribonucleotide biosynthesis during in vivo bacteriophage T4 DNA replication. Intrinsic control of synthesis of thymine and 5-hydroxymethylcytosine deoxyribonucleotides at precise ratio found in DNA.
The Journal of biological chemistry.
252(9):3019-27
[PMID] 323259.
1977
Role of deoxycytidylate deaminase in deoxyribonucleotide synthesis in bacteriophage T4 DNA replication.
The Journal of biological chemistry.
252(23):8603-8
[PMID] 925015.
1973
Noncovalent interactions in viruses: characterization of their role in the pH and thermally induced conformational changes in bromegrass mosaic virus.
Virology.
53(1):204-14
[PMID] 4706709.
1971
Measurement of temperature within the sample cell during sedimentation velocity experiments.
Analytical biochemistry.
40(2):267-80
[PMID] 5551540.
Grants
Jan 2020
– May 2021
Improving the activity of diamidines for potential therapeutic use for patients with myotonic dystrophy types 1 and 2
Role: Other
Funding: MYOTONIC DYSTROPHY FOU
Jan 2020
– Mar 2021
Improving the activity of diamidines for potential therapeutic use for patients with myotonic dystrophy types 1 and 2
Role: Other
Funding: MYOTONIC DYSTROPHY FOU
Feb 2019
– Dec 2021
Generation of Virus-Free Polio Vaccine Project: Phase IV, To progress Recombinant Poliovirus Virus-Like Particles (VLPs) into Commercially Viable Candidates
Role: Principal Investigator
Funding: UNIVERSITY OF LEEDS
via WORLD HLTH ORGANIZATION
Jan 2018
– Dec 2018
Characterization of Poliovirus VLPs Using a Mammalian Cell-Free System
Role: Principal Investigator
Funding: WORLD HLTH ORGANIZATION
Mar 2017
– Dec 2017
Characterization of Poliovirus VLPs Using a Mammalian Cell-Free System
Role: Principal Investigator
Funding: WORLD HLTH ORGANIZATION
Jul 2015
– Jun 2016
IPA AGREEMENT
Role: Principal Investigator
Funding: US DEPT OF VET AFF GAINESVILLE MED CTR
Apr 2014
– Mar 2017
IPA AGREEMENT
Role: Principal Investigator
Funding: US DEPT OF VET AFF GAINESVILLE MED CTR
Oct 2013
– Sep 2016
IPA for Brian Cain
Role: Principal Investigator
Funding: US DEPT OF VETERANS AFFAIRS
Contact Details
Phones:
- Business:
- (352) 294-8384
Emails:
- Business:
- flanegan@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100245
GAINESVILLE FL 32610 - Business Street:
-
CAMPUS BOX 100245
1600 SW ARCHER RD
R3-234A ARB
GAINESVILLE FL 326103003